Description
Introduction
SuperCut Universal Multiple Digestion Buffer is a Tris/BSA based universal restriction enzyme digestion buffer, It provides an optimal conditions for commercial available restriction enzymes mostly used in the gene cloning. High efficiency for restriction analysis can be demonstrated both at single and multiple digestions.
Ordering Information (Please indicate on ordering form)
500 μl Super Cut-Buffer (SB000S)
1 ml Super Cut-Buffer (SB0001)
5 ml Super Cut-Buffer (SB0005)
10 ml Super Cut-Buffer (SB0010)
Advantages
Convenient and economical
100% efficiency for single & multiple digestions
Double digestion using Super Cut-Buffer can be completed in 1 hour
Instructions for use
Super Cut-Buffer is 10X concentrated. Simply add a 1/10 volume to the final reaction mixture. Additional BSA is not required.
Example (total reaction volume 30 μl )
DNA 1 μg
Restriction Enzyme 1 unit
Super Cut-Buffer 3 μl
Sterilized distilled water up to 30 μl
Incubate at 37℃ for 1 hour
Stable a -20ºC for 1 year without freeze and thaw
For research use only.
Test Data
